Effect of Mg2+ on non-quantal acetylcholine release at the mouse neuromuscular junction
Language English Country Ireland Media print
Document type Comparative Study, Journal Article
PubMed
2812517
DOI
10.1016/0304-3940(89)90115-8
PII: 0304-3940(89)90115-8
Knihovny.cz E-resources
- MeSH
- Acetylcholine metabolism MeSH
- Diaphragm drug effects MeSH
- Electrophysiology MeSH
- Magnesium pharmacology MeSH
- Membrane Potentials MeSH
- Mice MeSH
- Neuromuscular Junction metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Acetylcholine MeSH
- Magnesium MeSH
The effects of extracellular concentrations of Mg2+ on the non-quantal release of acetylcholine (ACh) from nerve terminals was studied by extra- and intracellular electrophysiological methods. Anticholinesterase-treated mouse diaphragms were used in vitro. In the presence of Ca2+, the non-quantal release was maximal in the absence of Mg2+ and was inhibited by 3 mmol/l Mg2+. The inhibitory effect of Mg2+ was antagonized by ouabain and was absent in Ca2+-free (EGTA) solutions. The non-quantal release of ACh was found to be more sensitive to inhibition by Mg2+ than the quantal one which was measured as the amplitude of miniature endplate currents.
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