Oxygen increases the steady-state level of nitrate in denitrifying cells of Paracoccus denitrificans
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8961552
DOI
10.1111/j.1574-6968.1996.tb08572.x
PII: S0378109796004028
Knihovny.cz E-resources
- MeSH
- Biological Transport physiology MeSH
- Nitrates analysis metabolism MeSH
- Evaluation Studies as Topic MeSH
- Oxygen pharmacology MeSH
- Paracoccus denitrificans chemistry drug effects metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nitrates MeSH
- Oxygen MeSH
The levels of nitrate in denitrifying cells of Paracoccus denitrificans were determined by centrifugation through silicone oil into phosphoric acid and ion-exchange HPLC analysis of the cell lysates, using [14C]sucrose to correct for the trapped external medium. Introduction of oxygen brought about a significant upward shift in the intracellular nitrate concentration. This result calls into question the current thinking that oxygen blocks nitrate respiration primarily due to the inhibition of nitrate transport into the cell.
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