Properties of Syrian hamster cells transformed by human papillomavirus type 16
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9171456
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- DNA virů analýza MeSH
- křečci praví MeSH
- křeček rodu Mesocricetus MeSH
- lidé MeSH
- onkogenní proteiny virové genetika imunologie MeSH
- Papillomaviridae genetika imunologie MeSH
- Papillomavirus E7 - proteiny MeSH
- protilátky virové krev MeSH
- represorové proteiny * MeSH
- RNA virová analýza MeSH
- slezina cytologie imunologie MeSH
- transformované buněčné linie MeSH
- virová transformace buněk * MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA virů MeSH
- E6 protein, Human papillomavirus type 16 MeSH Prohlížeč
- oncogene protein E7, Human papillomavirus type 16 MeSH Prohlížeč
- onkogenní proteiny virové MeSH
- Papillomavirus E7 - proteiny MeSH
- protilátky virové MeSH
- represorové proteiny * MeSH
- RNA virová MeSH
Adult Syrian hamster kidney cells were transfected with a mixture of plasmids containing human papillomavirus type 16 (HPV16) E6/E7 open reading frames (ORFs), activated Ha-ras gene and neomycin resistance gene. From these cultures two lines were isolated which were oncogenic for newborn and 5-day-old but not for 3-week-old hamsters. Sublines oncogenic for 3-7-week-old hamsters were derived from tumours formed in animals inoculated within 5 days of birth. The cells contained HPV 16 DNA in an integrated form and HPV16 transcripts. The transcript patterns in low and high oncogenicity sublines were different. Very few tumour-bearing animals possessed antibodies reactive with E6- and E7-derived synthetic peptides. On the other hand a majority of these animals gave a positive reaction in the lymphoproliferation assay with either E6 and E7 peptides or extracts from the transformed cells.