Nucleolar coefficient and cytochemistry of human blood monocytes
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9188763
DOI
10.1016/s0024-3205(97)00235-x
PII: S002432059700235X
Knihovny.cz E-resources
- MeSH
- Cell Nucleolus chemistry MeSH
- Chromosomal Proteins, Non-Histone analysis MeSH
- Adult MeSH
- Phosphoproteins analysis MeSH
- Nuclear Proteins analysis MeSH
- Humans MeSH
- Monocytes chemistry cytology MeSH
- Nucleophosmin MeSH
- Nucleolin MeSH
- Nucleolus Organizer Region chemistry MeSH
- RNA-Binding Proteins * MeSH
- Ribonucleoproteins analysis MeSH
- RNA analysis MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromosomal Proteins, Non-Histone MeSH
- fibrillarin MeSH Browser
- Phosphoproteins MeSH
- Nuclear Proteins MeSH
- NPM1 protein, human MeSH Browser
- Nucleophosmin MeSH
- RNA-Binding Proteins * MeSH
- Ribonucleoproteins MeSH
- RNA MeSH
Nucleoli in peripheral blood monocytes represented mostly by micronucleoli were investigated by means of cytochemical procedures for the demonstration of RNA, proteins of silver stained nucleolus organizer region, nucleophosmin, nucleolin and fibrillarin. The values of the nucleolar coefficient (mean number of nucleoli per cell) were influenced by the procedure used for the visualization of nucleoli. The differences in the values of the nucleolar coefficient of monocytes in one and the same person using above mentioned procedures suggested that micronucleoli in blood monocytes represent heterogeneous nuclear structures and only some of them possess both RNA and characteristic nucleolar proteins. Therefore, the procedures used for the visualization of nucleoli must be always taken into consideration when the values of the nucleolar coefficient are evaluated.
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