Different progress of MDCK cell death after infection by two different influenza virus isolates
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články
PubMed
9253160
DOI
10.1002/(sici)1099-0844(19970601)15:2<87::aid-cbf726>3.0.co;2-6
PII: 10.1002/(SICI)1099-0844(19970601)15:2<87::AID-CBF726>3.0.CO;2-6
Knihovny.cz E-zdroje
- MeSH
- buněčná smrt fyziologie MeSH
- buněčné linie virologie MeSH
- fragmentace DNA účinky léků fyziologie MeSH
- hemaglutininy farmakologie MeSH
- infekce viry z čeledi Orthomyxoviridae patofyziologie MeSH
- kultivační média bez séra farmakologie MeSH
- kultivační média farmakologie MeSH
- Orthomyxoviridae fyziologie MeSH
- virus chřipky A fyziologie MeSH
- virus chřipky B fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hemaglutininy MeSH
- kultivační média bez séra MeSH
- kultivační média MeSH
The effect of influenza strains A (H3N2) and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with cytotoxicity were investigated in the absence and presence of 10 per cent calf serum. Virus replication, regardless of type A or B, caused earlier DNA fragmentation in comparison to non-infected cells in tissue culture. The results showed that the influenza B strain had a greater cytotoxic effect on MDCK cells than influenza A. A higher infection dose of influenza A virus accelerated the onset of apoptosis; conversely, a higher infection dose of influenza B virus delayed the onset of apoptosis. The absence of serum enhanced the progress of influenza-induced apoptosis in conditions in vitro.
Citace poskytuje Crossref.org
