Pouzitie metódy Western blotting na sérologickú diagnostiku enterovírusových infekcií. I. Detekcia anti-coxsackievírusových protilátok v referencných diagnostických sérach a v sérach experimentálne infikovaných mysí
[Western blotting in the serologic diagnosis of enterovirus infections. I. Detection of coxsackie virus antibodies in reference sera and sera of experimentally infected mice]
Jazyk slovenština Země Česko Médium print
Typ dokumentu anglický abstrakt, časopisecké články
PubMed
9264792
- MeSH
- coxsackie virózy diagnóza MeSH
- Enterovirus imunologie MeSH
- králíci MeSH
- myši MeSH
- protilátky virové krev MeSH
- sérologické testy MeSH
- western blotting * MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- protilátky virové MeSH
The introduction of rapid serological diagnosis of enterovirus infections is faced with the problem of inter-type cross-reactions. Therefore the method of choice is still the virus-neutralization test on cell cultures and on suckling mice resp., i.e. methods which are expensive and time consuming and demanding on laboratory equipment. The detection of antibodies against the Coxsackie virus by means of Western blotting revealed a specific reaction of commercial hyperimmune diagnostic rabbit as well as of sera obtained after oral infection of mice. The application of the method in the diagnosis of human enterovirus infections will be the subject of the subsequent part of this study.
Detection of coxsackie B virus infection using a rapid screening method
