Detection of coxsackie B virus infection using a rapid screening method
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
11200674
DOI
10.1007/bf02817452
Knihovny.cz E-zdroje
- MeSH
- antigeny virové imunologie MeSH
- coxsackie virózy krev virologie MeSH
- enterovirus B lidský imunologie MeSH
- lidé MeSH
- neutralizační testy MeSH
- protilátky virové krev MeSH
- senzitivita a specificita MeSH
- western blotting MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- antigeny virové MeSH
- protilátky virové MeSH
A Western blot method (WB) was adapted for rapid screening of antibodies against coxsackie virus B1-B6 in sera from patients with newly diagnosed insulin-dependent diabetes mellitus, myocarditis or febrile syndrome of suspected coxsackie viral aetiology. The use of a mixture of all 6 coxsackie virus B serotypes as the common antigen permitted a very rapid and inexpensive detection of antibody-positive sera for preliminary diagnosis and further detailed assay. Comparison of the results with those obtained in parallel run virus-neutralization tests showed a higher sensitivity and comparable specificity of WB.
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