Separation of cellular iron containing compounds by electrophoresis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9533565
DOI
10.1007/bf02789087
Knihovny.cz E-zdroje
- MeSH
- autoradiografie MeSH
- chelátory železa chemie MeSH
- deferoxamin chemie MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- senzitivita a specificita MeSH
- ultrafiltrace MeSH
- železo chemie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chelátory železa MeSH
- deferoxamin MeSH
- železo MeSH
High resolution separation of metalloproteins and other iron compounds based on native gel electrophoresis followed by 59Fe autoradiography is described. Lysates of mouse spleen erythroid cells metabolically labeled with 59Fe-transferrin were separated on 3-20% polyacrylamide gradient gels in the presence of Triton X100 and detected by autoradiography. In addition to ferritin and hemoglobin, several compounds characterized by their binding of iron under different conditions were described. Iron chelatable by desferrioxamine migrated in the region where several high-molecular weight compounds were detected by silver staining. The technique is nondissociative, allowing identification of iron compounds with the use of specific antibodies. Cellular iron transport and the action of iron chelators on specific cellular targets can be investigated in many small biological samples in parallel.
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