The serological surveillance of several groups of patients using antigens of Encephalitozoon hellem and E. cuniculi antibodies to microsporidia in patients
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9684320
Knihovny.cz E-zdroje
- MeSH
- antigeny protozoální izolace a purifikace MeSH
- Cercopithecus aethiops MeSH
- demence imunologie parazitologie MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- ELISA MeSH
- Encephalitozoon cuniculi imunologie izolace a purifikace MeSH
- Encephalitozoon imunologie izolace a purifikace MeSH
- HIV infekce imunologie parazitologie MeSH
- lidé MeSH
- Microsporidia imunologie izolace a purifikace MeSH
- myši MeSH
- protilátky protozoální krev MeSH
- pyelonefritida imunologie parazitologie MeSH
- rizikové faktory MeSH
- sentinelová surveillance MeSH
- těhotenství MeSH
- Vero buňky MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny protozoální MeSH
- protilátky protozoální MeSH
This study was undertaken to attempt to identify correlations between microsporidial seroprevalence data in man, clinical diseases and groups of people at the risk of HIV/AIDS infection. Groups of patients were selected according to the predilection of members of the genus Encephalitozoon for nervous and kidney tissue. Female prostitutes and alcohol and intravenous drug abusers were selected as groups at risk of HIV/AIDS infections. A total of 401 samples of human sera were examined for the presence of antimicrosporidial IgG antibodies by ELISA test with a titre of 600 considered borderline positivity. The highest occurrence of antimicrosporidial antibodies was found in the groups of alcohol abusers (16% from 43 patients), intravenous drug abusers (11% from 9 patients) and prostitutes (10% from 80 women) for E. cuniculi antigen and in the groups of psychiatric patients (14% from 44 patients), malaria patients (11% from 38 patients) and alcohol abusers (7% from 43 patients) for E. hellem antigen. The occurrence of specific antibodies of the six examined diagnostic units (glomerulonephritis chronica, pyelonephritis chronica, schizophrenia, dementia, multiple sclerosis and cerebral stroke) was statistically significant only in patients with pyelonephritis chronica and dementia (p < 0.05). No cases of microsporidial infection were found among the female prostitutes by parasitological examination, although one case of giardiasis was identified. Sera of patients with high anti-E. cuniculi and anti-E. hellem antibodies (titres in ELISA of 600 and above) were confirmed by Western blot using E. cuniculi and E. hellem polypeptides, respectively. These results suggest that the examined patients could show residual antibodies from past or latent infections.
Latent microsporidial infection in immunocompetent individuals - a longitudinal study
Unapparent microsporidial infection among immunocompetent humans in the Czech Republic
Seropositivity for Enterocytozoon bieneusi, Czech Republic
