Comparison of oriented and random antibody immobilization in immunoaffinity chromatography of cytokinins
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
9691302
DOI
10.1016/s0021-9673(98)00210-6
PII: S0021-9673(98)00210-6
Knihovny.cz E-zdroje
- MeSH
- celulosa MeSH
- chromatografie afinitní MeSH
- cytokininy chemie MeSH
- imunochemie MeSH
- imunoglobulin G chemie MeSH
- molekulární konformace MeSH
- oxidace-redukce MeSH
- protilátky chemie MeSH
- semena rostlinná chemie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- celulosa MeSH
- cytokininy MeSH
- imunoglobulin G MeSH
- protilátky MeSH
Immunosorbents for the plant hormones cytokinins prepared by random antibody immobilization (to Affi-Gel 10) and by oriented approach via oxidized carbohydrate moieties on the Fc region (to Affi-Gel Hz or hydrazide derivative of Perloza MT 200) have been compared. Both approaches yielded immunosorbents with high dynamic capacity (ca. 5-10 nmol ml gel-1). Oriented antibody immobilization did not exhibit crucial effects in the case of low-molecular-mass cytokinins. Antibodies immobilized via a spacer to Affi-Gel 10 have probably enough conformational freedom to enable good accessibility to cytokinins. The sorbents were used in analysis of endogenous cytokinins in maize seeds. In phosphatase treated samples trans-zeatin and its riboside were predominant.
Citace poskytuje Crossref.org
