Preferential silver reaction of nucleolar regions adjacent to fibrillar centers in ring shaped nucleoli of leukemic lymphocytes
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- B-lymfocyty chemie ultrastruktura MeSH
- barvení stříbrem metody MeSH
- buněčné jadérko chemie ultrastruktura MeSH
- chronická lymfatická leukemie patologie MeSH
- fixace tkání MeSH
- HeLa buňky chemie ultrastruktura MeSH
- jaderné proteiny analýza MeSH
- kyselina chlorovodíková chemie MeSH
- lidé MeSH
- organizátor jadérka chemie ultrastruktura MeSH
- stříbro chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- jaderné proteiny MeSH
- kyselina chlorovodíková MeSH
- stříbro MeSH
Silver stained proteins (SSPs) characteristic for interphasic nucleolus organizer regions (NORs) associated with fibrillar centers (FCs) and adjacent nucleolar regions of ring shaped nucleoli in leukemic lymphocytes exhibit a different sensitivity to the mild acid extraction including that with HCl. Such extractions permit a preferential visualization of fibrillar centers adjacent regions (FCARs) which are believed to represent sites of the ribosomal RNA (rRNA) transcription. The resistance of SSPs in FCARs to the extraction with HCl seems to be due to their binding to other components present in these regions. The extractibility of SSPs with HCl was influenced by the fixatives used. The largest resistance of SSPs to the extraction with HCl was noted after fixation with glutaraldehyde. In contrast, the largest extractibility of these proteins was observed after fixation with unbuffered formaldehyde.
Citace poskytuje Crossref.org
