A proposal for the reliable culture of Borrelia burgdorferi from patients with chronic Lyme disease, even from those previously aggressively treated
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, multicentrická studie
PubMed
9861561
DOI
10.1007/bf02770837
Knihovny.cz E-zdroje
- MeSH
- bakteriemie diagnóza farmakoterapie mikrobiologie MeSH
- bakteriologické techniky normy MeSH
- Borrelia burgdorferi komplex izolace a purifikace ultrastruktura MeSH
- chronická nemoc MeSH
- diferenciální diagnóza MeSH
- lidé MeSH
- lymeská nemoc diagnóza farmakoterapie mikrobiologie MeSH
- prediktivní hodnota testů MeSH
- recidiva MeSH
- reprodukovatelnost výsledků MeSH
- studie případů a kontrol MeSH
- terapie neúspěšná MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- Geografické názvy
- Česká republika MeSH
- City of New York MeSH
- Connecticut MeSH
- Texas MeSH
Since culture of Borrelia burgdorferi from patients with chronic Lyme disease has been an extraordinarily rare event, clarification of the nature of the illness and proving its etiology as infectious have been difficult. A method for reliably and reproducibly culturing B. burgdorferi from the blood of patients with chronic Lyme disease was therefore sought by making a controlled blood culture trial studying 47 patients with chronic Lyme disease. All had relapsed after long-term oral and intravenous antibiotics. 23 patients with other chronic illness formed the control group. Positive cultures were confirmed by fluorescent antibody immuno-electron microscopy using monoclonal antibody directed against Osp A, and Osp A PCR. 43/47 patients (91%) cultured positive. 23/23 controls (100%) cultured negative. Although persistent infection has been, to date, strongly suggested in chronic Lyme disease by positive PCR and antigen capture, there are major problems with these tests. This new method for culturing B. burgdorferi from patients with chronic Lyme disease certainly defines the nature of the illness and establishes that it is of chronic infectious etiology. This discovery should help to reestablish the gold standard in laboratory diagnosis of Lyme disease.
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