A multidrug-resistant tuberculosis microepidemic caused by genetically closely related Mycobacterium tuberculosis strains
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10405429
PubMed Central
PMC85324
DOI
10.1128/jcm.37.8.2715-2716.1999
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial analysis genetics MeSH
- DNA Fingerprinting MeSH
- Disease Outbreaks MeSH
- Humans MeSH
- Drug Resistance, Multiple MeSH
- Mycobacterium tuberculosis drug effects genetics pathogenicity MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Tuberculosis epidemiology microbiology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- DNA, Bacterial MeSH
IS6110 DNA fingerprinting was used to characterize an outbreak of multidrug-resistant tuberculosis in 21 individuals (17 males and 4 females) living in or roaming among four distantly separated areas in the Czech Republic. The restriction fragment length polymorphism (RFLP) analysis separated the collected Mycobacterium tuberculosis strains into group A, including 14 patients with six IS6110 copies, and group B, with 7 patients displaying highly similar RFLP patterns but with two additional IS6110 bands. A switch from pattern A to pattern B was observed in one patient, and the subsequent detection of subclone B in seven more individuals has been explained by the instability of DNA genotypes caused by transposition of IS6110 elements.
See more in PubMed
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