Indigenous plasmids in a production line of strains for penicillin G acylase derived from Escherichia coli W
Language English Country United States Media print
Document type Journal Article
PubMed
10664880
DOI
10.1007/bf02818544
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial MeSH
- Escherichia coli enzymology genetics MeSH
- Phenotype MeSH
- Microbial Sensitivity Tests MeSH
- Penicillin Amidase biosynthesis genetics MeSH
- Plasmids genetics MeSH
- Industrial Microbiology MeSH
- Recombinant Proteins biosynthesis MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Penicillin Amidase MeSH
- Recombinant Proteins MeSH
Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the strain RE3 without plasmid pRK1 and the plasmid-less strain cERE3 were isolated. Indigenous plasmids did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.
