Rpg1p, the subunit of the Saccharomyces cerevisiae eIF3 core complex, is a microtubule-interacting protein
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10706778
DOI
10.1002/(sici)1097-0169(200003)45:3<235::aid-cm6>3.0.co;2-i
PII: 10.1002/(SICI)1097-0169(200003)45:3<235::AID-CM6>3.0.CO;2-I
Knihovny.cz E-resources
- MeSH
- Eukaryotic Initiation Factor-3 MeSH
- Fluorescent Antibody Technique MeSH
- Peptide Initiation Factors genetics metabolism MeSH
- Microtubules metabolism MeSH
- Prokaryotic Initiation Factor-3 MeSH
- Cell Cycle Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae Proteins * MeSH
- Saccharomyces cerevisiae metabolism ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Eukaryotic Initiation Factor-3 MeSH
- Peptide Initiation Factors MeSH
- Prokaryotic Initiation Factor-3 MeSH
- Cell Cycle Proteins MeSH
- RPG1 protein, S cerevisiae MeSH Browser
- Saccharomyces cerevisiae Proteins * MeSH
The essential gene RPG1/TIF32 of Saccharomyces cerevisiae encodes the 110-kDa subunit of the translation initiation factor 3 (eIF3) core complex. In this study, the Rpg1p-specific monoclonal antibody PK1/1 was used to analyse the cellular distribution of Rpg1p by epifluorescence and confocal laser scanning microscopy (CLSM). In budded cells, a portion of Rpg1p was obviously co-localised with microtubules. In addition, CLSM revealed an accumulation of Rpg1p in a patch at the very end of cytoplasmic microtubules reaching the bud tip. A punctate fluorescence pattern was typical for separated unbudded cells. Distribution of Rpg1p was confirmed using a strain expressing exclusively a hemaglutinin-tagged version of Rpg1p. In nocodazole-treated cells, the pattern of the PK1/1 staining was disturbed. No staining was observed in Rpg1p-depleted cells. In vitro experiments revealed that Rpg1p was specifically co-immunoprecipitated with alpha-tubulin from the yeast cell free extract and this observation was further supported by showing that Rpg1p co-sedimented with hog brain microtubules. We conclude that Rpg1p is a microtubule-interacting protein that indicates an interesting connection between the translation initiation machinery and cytoskeleton in yeast Saccharomyces cerevisiae.
References provided by Crossref.org
General and molecular microbiology and microbial genetics in the IM CAS
The fission yeast ortholog of eIF3a subunit is not functional in Saccharomyces cerevisiae
