Growth and differentiation of the vascular smooth muscle and endothelial cells cultured on fluorine ion-implanted polystyrene
Language English Country Slovakia Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10707836
Knihovny.cz E-resources
- MeSH
- Actins metabolism MeSH
- Cell Differentiation MeSH
- Cell Division MeSH
- Cell Culture Techniques methods MeSH
- Antigens, CD metabolism MeSH
- Endothelium, Vascular cytology immunology metabolism MeSH
- Fluorine MeSH
- Immunohistochemistry MeSH
- Integrin alphaV MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Intercellular Adhesion Molecule-1 metabolism MeSH
- Polystyrenes MeSH
- Cattle MeSH
- Muscle, Smooth, Vascular cytology immunology metabolism MeSH
- Talin metabolism MeSH
- Vimentin metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Antigens, CD MeSH
- Fluorine MeSH
- Integrin alphaV MeSH
- Intercellular Adhesion Molecule-1 MeSH
- Polystyrenes MeSH
- Talin MeSH
- Vimentin MeSH
The rat vascular (SMCs) and bovine endothelial cells (BECs) were cultured on conventional or fluorine ion-implanted polystyrene (5 x 10(12) and 5 x 10(14) fluorine ions/cm2). The cells grown on the implanted growth supports showed better adherence, higher volume and higher total protein content. The immunocytochemical analysis revealed that SMCs contained more of the cytoskeletal vimentin and the vascular SMC-specific alpha-actin as well as several cell adhesion-mediating molecules (vinculin, talin, alpha(v)-integrin and ICAM-1). In BECs, only the content of vimentin and talin increased, while expression of ICAM-1 was unchanged. The data suggest that cells on the ion implanted polymers could be more viable and that increased expression of some adhesion molecules mediating interactions with the host immune system is cell type-dependent.
