Growth and differentiation of the vascular smooth muscle and endothelial cells cultured on fluorine ion-implanted polystyrene
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10707836
Knihovny.cz E-zdroje
- MeSH
- aktiny metabolismus MeSH
- buněčná diferenciace MeSH
- buněčné dělení MeSH
- buněčné kultury metody MeSH
- CD antigeny metabolismus MeSH
- cévní endotel cytologie imunologie metabolismus MeSH
- fluor MeSH
- imunohistochemie MeSH
- integrin alfaV MeSH
- krysa rodu Rattus MeSH
- kultivované buňky MeSH
- mezibuněčná adhezivní molekula-1 metabolismus MeSH
- polystyreny MeSH
- skot MeSH
- svaly hladké cévní cytologie imunologie metabolismus MeSH
- talin metabolismus MeSH
- vimentin metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aktiny MeSH
- CD antigeny MeSH
- fluor MeSH
- integrin alfaV MeSH
- mezibuněčná adhezivní molekula-1 MeSH
- polystyreny MeSH
- talin MeSH
- vimentin MeSH
The rat vascular (SMCs) and bovine endothelial cells (BECs) were cultured on conventional or fluorine ion-implanted polystyrene (5 x 10(12) and 5 x 10(14) fluorine ions/cm2). The cells grown on the implanted growth supports showed better adherence, higher volume and higher total protein content. The immunocytochemical analysis revealed that SMCs contained more of the cytoskeletal vimentin and the vascular SMC-specific alpha-actin as well as several cell adhesion-mediating molecules (vinculin, talin, alpha(v)-integrin and ICAM-1). In BECs, only the content of vimentin and talin increased, while expression of ICAM-1 was unchanged. The data suggest that cells on the ion implanted polymers could be more viable and that increased expression of some adhesion molecules mediating interactions with the host immune system is cell type-dependent.
