Determination of finasteride in human plasma by liquid-liquid extraction and high-performance liquid chromatography
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
- MeSH
- finasterid krev farmakokinetika MeSH
- inhibitory enzymů krev farmakokinetika MeSH
- lidé MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- spektrofotometrie ultrafialová MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- finasterid MeSH
- inhibitory enzymů MeSH
A high-performance liquid chromatographic method for the quantitation of finasteride in human plasma is presented. The method is based on liquid-liquid extraction with hexane-isoamylalcohol (98:2, v/v) and reversed-phase chromatography with spectrophotometric detection at 210 nm. The mobile phase consists of acetonitrile-15 mM potassium dihydrogenphosphate (40:60, v/v). Clobazam is used as the internal standard. The limit of quantitation is 4 ng/ml and the calibration curve is linear up to 300 ng/ml. Within-day and between-day precision expressed by relative standard deviation is less than 5% and inaccuracy does not exceed 8%. The assay was used for pharmacokinetic studies.
Citace poskytuje Crossref.org
