Cellular activity of murine phagocytes isolated from peripheral blood by a discontinuous gradient
Jazyk angličtina Země Česko Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
10730853
Knihovny.cz E-zdroje
- MeSH
- centrifugace - gradient hustoty MeSH
- diatrizoát MeSH
- fagocytóza MeSH
- fagocyty cytologie fyziologie MeSH
- Ficoll MeSH
- luminiscenční měření MeSH
- monocyty cytologie fyziologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- neutrofily cytologie fyziologie MeSH
- separace buněk metody MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- diatrizoát MeSH
- Ficoll MeSH
A mixture of Ficoll 400 and sodium diatrizoate (Hypaque) at a density of 1.077 g/ml has been used to isolate the mononuclear cells from the remaining haematic cells. A simple, inexpensive and classical method was established to obtain substantially erythrocyte-free polymorphonuclear cell preparations from mouse peripheral blood, using a mixture of the same substances but at a density of 1.119 g/ml. This method along with that at a density of 1.077 g/ml allows two cellular bands to appear which contain mononuclear and polymorphonuclear (PMN) cells, respectively. Using this method, the counts of monocytes isolated from peripheral blood are significantly greater than those obtained by a one-step Ficoll-Hypaque procedure. On the contrary, the counts of PMN cells are significantly smaller than when sedimentation in dextran (6% solution) is used after gradient centrifugation. In this paper, chemiluminescence assay has been used to analyze the possible variations in phagocytic activity of cells isolated by both procedures, since it appears to be one of the most sensitive methods available for this purpose. The results obtained show a slightly greater activation in monocytes and PMN cells isolated by one-step Ficoll-Hypaque procedure, in comparison with another method which uses both Ficoll-Hypaque 1077 and Ficoll-Hypaque 1119, although statistical differences were not significant.