Aggregated forms of heparin-binding and non-heparin-binding proteins of boar seminal plasma and their binding properties
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
10730888
Knihovny.cz E-resources
- MeSH
- Chondroitin Sulfates metabolism MeSH
- Chromatography, Affinity MeSH
- Heparin metabolism MeSH
- Sperm Capacitation MeSH
- Hyaluronic Acid metabolism MeSH
- Macromolecular Substances MeSH
- Molecular Weight MeSH
- Polysaccharides metabolism MeSH
- Swine metabolism MeSH
- Proteins isolation & purification metabolism MeSH
- Sequence Analysis, Protein MeSH
- Dextran Sulfate metabolism MeSH
- Semen chemistry MeSH
- Body Fluids chemistry MeSH
- Protein Binding MeSH
- Chromatography, High Pressure Liquid MeSH
- Zona Pellucida metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Chondroitin Sulfates MeSH
- fucoidan MeSH Browser
- Heparin MeSH
- Hyaluronic Acid MeSH
- Macromolecular Substances MeSH
- Polysaccharides MeSH
- Proteins MeSH
- Dextran Sulfate MeSH
Boar seminal plasma proteins were separated by affinity chromatography on immobilized heparin into two portions: heparin-binding (H+) and non-heparin-binding (H-) proteins. Gel chromatography of the H+ portion yielded four main protein fractions of >150, 45, 30 and 20 kDa, while that of the H- portion resulted in the separation into three main protein fractions of >150, 30 and 20 kDa. HPLC analysis and N-terminal sequencing used to characterize the composition of the protein fractions obtained by gel chromatography revealed that all consisted of low (12-16 kDa) molecular weight components: the H+ fraction consisted of DQH sperm protein, AQN and AWN spermadhesins whereas the H- fraction consisted of PSPI and PSPII spermadhesins. The high molecular weight values of fractions obtained by gel chromatography thus suggest that the proteins are present in boar seminal plasma in the form of aggregates. Interactions of individual boar seminal plasma proteins and their aggregates present in the H+ and H- fractions with acid polysaccharides were estimated.
Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation
