A total of 2816 unfed adults nymphs of Ixodes ricinus ticks were collected from vegetation in Kosice (Eastern Slovakia) from 1994 to 1997. Prevalence of Borrelia burgdorferi s. l. in I. ricinus ticks, detected by dark field microscopy, varied and depended upon the year and the habitat of the collected ticks. The lowest prevalence was observed in 1994 (4.8%). During 1995 it increased to 17.2% and during the next two years decreased to 15.5% and 14.2%. The rate of infection varied from 2.1 to 23.3% within 10 examined habitats of the Kosice area. A different value of relative density of ticks was observed in various habitats. It ranged from 9-212 ticks per collecting hour within one flagged area (600 m2) which is 1.5-35.5 ticks per 100 m2. Eight isolates were obtained from the infected ticks. Electrophoresis and immunoblotting with 6 monoclonal antibodies were used for the identification of Borrelia strains. Three tick isolates were identified as B. burgdorferi s. s. and the other three isolates were found to be B. garinii. One strain reacted as a mixed culture of B. burgdorferi s. s., and B. garinii. The strain originated from the Vihorlat Mountains habitat and was detected by PCR-SSCP as B. burgdorferi s. s. with a small amount of B. afzelii. The obtained results emphasize the epidemiological importance not only of B. garinii and B. afzelii but also of B. burgdorferi s. s. in Central Europe.

Institute of Parasitology Academy of Sciences of the Czech Republic Ceské Budejovice

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Eur J Epidemiol. 1997 Dec;13(8):951-7 PubMed

Nature. 1970 Aug 15;227(5259):680-5 PubMed

Orv Hetil. 1991 Jan 20;132(3):129-30, 133-4 PubMed

Przegl Epidemiol. 1996;50(4):383-6 PubMed

Ann Agric Environ Med. 1998;5(1):25-30 PubMed

Int J Syst Bacteriol. 1992 Jul;42(3):370-7 PubMed

Bratisl Lek Listy. 1990 Apr;91(4):251-66 PubMed

Parazitologiia. 1990 Jul-Aug;24(4):257-67 PubMed

Zentralbl Bakteriol. 1997 Oct;286(3):355-61 PubMed

Cent Eur J Public Health. 1995 Aug;3(3):134-7 PubMed

Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PubMed

Int J Syst Bacteriol. 1997 Oct;47(4):921-5 PubMed

Int J Syst Bacteriol. 1992 Jul;42(3):378-83 PubMed

Folia Parasitol (Praha). 1990;37(4):359-62 PubMed

J Infect Dis. 1985 Sep;152(3):478-84 PubMed

Folia Parasitol (Praha). 1998;45(1):73-4 PubMed

Folia Parasitol (Praha). 1998;45(1):67-72 PubMed

Int J Syst Bacteriol. 1997 Oct;47(4):926-32 PubMed

Zentralbl Bakteriol. 1992 Jun;277(1):28-33 PubMed

J Infect Dis. 1996 Aug;174(2):424-6 PubMed

Zentralbl Bakteriol Mikrobiol Hyg A. 1989 Jan;270(3):434-40 PubMed

Anal Biochem. 1976 May 7;72:248-54 PubMed

Cesk Epidemiol Mikrobiol Imunol. 1990 Jun;39(3):166-70 PubMed

Res Microbiol. 1992 Jul-Aug;143(6):583-96 PubMed

Science. 1982 Jun 18;216(4552):1317-9 PubMed

Scand J Infect Dis. 1993;25(4):441-8 PubMed

Zentralbl Bakteriol. 1990 Apr;272(4):426-36 PubMed

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Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in ixodes ricinus ticks from the Czech Republic