Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10958385
PubMed Central
PMC1781746
DOI
10.1080/096293500411587
Knihovny.cz E-zdroje
- MeSH
- Aggregatibacter actinomycetemcomitans imunologie MeSH
- agresivní parodontitida imunologie MeSH
- dospělí MeSH
- Escherichia coli imunologie MeSH
- imunoglobulin A metabolismus MeSH
- imunoglobulin G metabolismus MeSH
- imunoglobulin M metabolismus MeSH
- interferon gama biosyntéza MeSH
- interleukin-4 biosyntéza MeSH
- kultivované buňky MeSH
- leukocyty mononukleární cytologie imunologie MeSH
- lidé MeSH
- mladiství MeSH
- nukleární rodina MeSH
- Th1 buňky imunologie MeSH
- Th2 buňky imunologie MeSH
- věk při počátku nemoci MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- imunoglobulin A MeSH
- imunoglobulin G MeSH
- imunoglobulin M MeSH
- interferon gama MeSH
- interleukin-4 MeSH
Early onset periodontitis (EOP) is a chronic inflammatory periodontal disease with a strong genetic link affecting individuals aged 17 to 25. In the familial studies we tested the hypothesis about the role of Th1 and Th2 cytokines in the pathogenesis of EOP disease. The study involved 6 individuals with EOP disease and their 6 siblings with healthy periodontium. Actinobacillus actinomycetemcomitans (A. a), a bacterium typical for EOP, was detected in all people studied. Th1 and Th2 cytokine production was measured after in vitro stimulation. Peripheral blood mononuclear cells (PBMC) were isolated and cultivated for 24 h and 7 days with PWM, A. a. or Escherichia coli. The levels of IL-4, IFN-gamma, IgA, IgG and IgM were measured by ELISA methods. After in vitro stimulation of PBMC, a significantly higher production of IL-4 and significantly lower production of IFN-gamma were found in the group of patients compared with their healthy siblings. The increased level of IL-4 in patients was in good agreement with an increased level of IgM after stimulation of lymphocytes with E. coli. These results support Seymour's hypothesis according to which patients with progressive disease primarily activate Th2 lymphocytes while non-susceptible individuals activate Th1 lymphocytes.
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