Stimulation of human blood lymphocyte by different polyclonal B cell activators of bacterial and plant origin: production of IgM, IgG and IgA estimated by the ELISA method
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
3891552
DOI
10.1007/bf02923518
Knihovny.cz E-zdroje
- MeSH
- Actinomyces imunologie MeSH
- aktivace lymfocytů účinky léků MeSH
- B-lymfocyty imunologie MeSH
- dospělí MeSH
- ELISA MeSH
- Escherichia coli imunologie MeSH
- flagelin farmakologie MeSH
- imunoglobulin A biosyntéza MeSH
- imunoglobulin G biosyntéza MeSH
- imunoglobulin M biosyntéza MeSH
- lidé MeSH
- mitogeny líčidla amerického farmakologie MeSH
- mitogeny farmakologie MeSH
- Nocardia imunologie MeSH
- tvorba protilátek * účinky léků MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- flagelin MeSH
- imunoglobulin A MeSH
- imunoglobulin G MeSH
- imunoglobulin M MeSH
- mitogeny líčidla amerického MeSH
- mitogeny MeSH
Lymphocytes isolated from peripheral blood of healthy donors were stimulated in vitro with pokeweed mitogen, concanavalin A, flagellin, Nocardia delipidated cell mitogen (NDCM) and heat-killed bacteria Escherichia coli and Actinomyces viscosus. A simple and sensitive technique, enzyme-linked immunosorbent assay (ELISA) was used for the detection of nanogram levels of IgM, IgA and IgC in media from lymphocyte cultures after polyclonal stimulation, Pokeweed mitogen, NDCM and E. coli were shown to stimulate a high production of IgM; after stimulation with A. viscosus a higher production of IgA was detected. No immunoglobulin production was observed after stimulation with polymerized flagellin.
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