Polyclonal immunoglobulin response of thymic, hepatic and splenic lymphocytes from fetal, germ-free and conventionally reared pigs to different B-cell activators
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8763157
DOI
10.1007/bf02814751
Knihovny.cz E-zdroje
- MeSH
- aktivace lymfocytů * MeSH
- B-lymfocyty účinky léků imunologie MeSH
- bakteriální proteiny * MeSH
- chaperon hsp60 MeSH
- chaperoniny imunologie MeSH
- ELISA MeSH
- gnotobiologické modely imunologie MeSH
- játra cytologie embryologie růst a vývoj imunologie MeSH
- lipopolysacharidy imunologie MeSH
- mitogeny líčidla amerického imunologie MeSH
- mitogeny imunologie MeSH
- organické látky MeSH
- orgánové kultury - kultivační techniky MeSH
- plod imunologie MeSH
- prasata embryologie růst a vývoj imunologie MeSH
- slezina cytologie imunologie MeSH
- thymus cytologie embryologie růst a vývoj imunologie MeSH
- tvorba protilátek * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny * MeSH
- chaperon hsp60 MeSH
- chaperoniny MeSH
- heat-shock protein 65, Mycobacterium MeSH Prohlížeč
- lipopolysacharidy MeSH
- mitogeny líčidla amerického MeSH
- mitogeny MeSH
- Nocardia delipidated cell mitogen MeSH Prohlížeč
- organické látky MeSH
Immunoglobulin (Ig) response to different polyclonal B-cell activators was measured by ELISA in cell culture media of thymocytes, splenocytes and liver cells isolated from pig fetuses, 8-d-old germ-free piglets and conventionally reared pigs. Both in fetal and in postnatal life polyclonally stimulated lymphocytes were found to produce predominantly the IgM isotype; the first IgM formation was detected in 50-d-old fetal liver (gestation in pigs lasts 114 d). Surprisingly, 73-d-old fetal thymic cells were shown to be induced to Ig synthesis and secretion. In contrast to splenocytes of the same age, which secreted exclusively IgM, fetal thymocytes produced IgM, IgG and IgA. Polyclonally stimulated splenic cells as compared with thymic cells started to produce IgA later in fetal ontogeny, whereas the IgG response was not detectable in splenic cell culture media during the whole embryonal development and appeared only after birth. The earliest and the highest Ig stimulation was found after cultivation of lymphocytes with Nocardia delipidated cell mitogen. Interestingly, the moderate stimulatory effect of 65-kDa heat shock protein (Hsp-65) in polyclonal IgM response of fetal splenocytes was observed. We showed that thymic B lymphocytes represent probably the first maturing B cell population detectable in fetal life, which is able to differentiate after polyclonal stimulation into IgM as well as IgA and IgG producing cells.
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