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Polyclonal immunoglobulin response of thymic, hepatic and splenic lymphocytes from fetal, germ-free and conventionally reared pigs to different B-cell activators

Folia microbiologica. 1995 ; 40 (4) : 421-30.

Folia Microbiol (Praha)
ISSN 0015-5632
Source

Language English Country United States Media print

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid08763157

PubMed 8763157
DOI 10.1007/bf02814751
Knihovny.cz E-resources

MeSH
Lymphocyte Activation * MeSH
B-Lymphocytes drug effects immunology MeSH
Bacterial Proteins * MeSH
Chaperonin 60 MeSH
Chaperonins immunology MeSH
Enzyme-Linked Immunosorbent Assay MeSH
Germ-Free Life immunology MeSH
Liver cytology embryology growth & development immunology MeSH
Lipopolysaccharides immunology MeSH
Pokeweed Mitogens immunology MeSH
Mitogens immunology MeSH
Organic Chemicals MeSH
Organ Culture Techniques MeSH
Fetus immunology MeSH
Swine embryology growth & development immunology MeSH
Spleen cytology immunology MeSH
Thymus Gland cytology embryology growth & development immunology MeSH
Antibody Formation * MeSH
Animals MeSH
Check Tag
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
Bacterial Proteins * MeSH
Chaperonin 60 MeSH
Chaperonins MeSH
heat-shock protein 65, Mycobacterium MeSH Browser
Lipopolysaccharides MeSH
Pokeweed Mitogens MeSH
Mitogens MeSH
Nocardia delipidated cell mitogen MeSH Browser
Organic Chemicals MeSH

Immunoglobulin (Ig) response to different polyclonal B-cell activators was measured by ELISA in cell culture media of thymocytes, splenocytes and liver cells isolated from pig fetuses, 8-d-old germ-free piglets and conventionally reared pigs. Both in fetal and in postnatal life polyclonally stimulated lymphocytes were found to produce predominantly the IgM isotype; the first IgM formation was detected in 50-d-old fetal liver (gestation in pigs lasts 114 d). Surprisingly, 73-d-old fetal thymic cells were shown to be induced to Ig synthesis and secretion. In contrast to splenocytes of the same age, which secreted exclusively IgM, fetal thymocytes produced IgM, IgG and IgA. Polyclonally stimulated splenic cells as compared with thymic cells started to produce IgA later in fetal ontogeny, whereas the IgG response was not detectable in splenic cell culture media during the whole embryonal development and appeared only after birth. The earliest and the highest Ig stimulation was found after cultivation of lymphocytes with Nocardia delipidated cell mitogen. Interestingly, the moderate stimulatory effect of 65-kDa heat shock protein (Hsp-65) in polyclonal IgM response of fetal splenocytes was observed. We showed that thymic B lymphocytes represent probably the first maturing B cell population detectable in fetal life, which is able to differentiate after polyclonal stimulation into IgM as well as IgA and IgG producing cells.

Department of Immunology and Gnotobiology Academy of Sciences of the Czech Republic Prague Czech Republic

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