Stimulation of human blood lymphocyte by different polyclonal B cell activators of bacterial and plant origin: production of IgM, IgG and IgA estimated by the ELISA method
Language English Country United States Media print
Document type Journal Article
PubMed
3891552
DOI
10.1007/bf02923518
Knihovny.cz E-resources
- MeSH
- Actinomyces immunology MeSH
- Lymphocyte Activation drug effects MeSH
- B-Lymphocytes immunology MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Escherichia coli immunology MeSH
- Flagellin pharmacology MeSH
- Immunoglobulin A biosynthesis MeSH
- Immunoglobulin G biosynthesis MeSH
- Immunoglobulin M biosynthesis MeSH
- Humans MeSH
- Pokeweed Mitogens pharmacology MeSH
- Mitogens pharmacology MeSH
- Nocardia immunology MeSH
- Antibody Formation * drug effects MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Flagellin MeSH
- Immunoglobulin A MeSH
- Immunoglobulin G MeSH
- Immunoglobulin M MeSH
- Pokeweed Mitogens MeSH
- Mitogens MeSH
Lymphocytes isolated from peripheral blood of healthy donors were stimulated in vitro with pokeweed mitogen, concanavalin A, flagellin, Nocardia delipidated cell mitogen (NDCM) and heat-killed bacteria Escherichia coli and Actinomyces viscosus. A simple and sensitive technique, enzyme-linked immunosorbent assay (ELISA) was used for the detection of nanogram levels of IgM, IgA and IgC in media from lymphocyte cultures after polyclonal stimulation, Pokeweed mitogen, NDCM and E. coli were shown to stimulate a high production of IgM; after stimulation with A. viscosus a higher production of IgA was detected. No immunoglobulin production was observed after stimulation with polymerized flagellin.
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