The complete primary structure of the str operon of Bacillus stearothermophilus was determined. It was established that the operon is a five-gene transcriptional unit: 5'-ybxF (unknown function; homology to eukaryotic ribosomal protein L30)-rpsL (S12)-rpsG (S7)-fus (elongation factor G [EF-G])-tuf (elongation factor Tu [EF-Tu])-3'. The main operon promoter (strp) was mapped upstream of ybxF, and its strength was compared with the strength of the tuf-specific promoter (tufp) located in the fus-tuf intergenic region. The strength of the tufp region to initiate transcription is about 20-fold higher than that of the strp region, as determined in chloramphenicol acetyltransferase assays. Deletion mapping experiments revealed that the different strengths of the promoters are the consequence of a combined effect of oppositely acting cis elements, identified upstream of strp (an inhibitory region) and tufp (a stimulatory A/T-rich block). Our results suggest that the oppositely adjusted core promoters significantly contribute to the differential expression of the str operon genes, as monitored by the expression of EF-Tu and EF-G.

Department of Protein Biosynthesis Institute of Molecular Genetics Academy of Sciences of the Czech Republic 166 37 Prague 6 Czech Republic

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Bacterial nanotubes as a manifestation of cell death

The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes

YbxF, a protein associated with exponential-phase ribosomes in Bacillus subtilis

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AJ133759, AJ133760, AJ246558, AJ249559