Selective antioxidant enzymes during ischemia/reperfusion in myocardial infarction
Language English Country Czech Republic Media print
Document type Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
PubMed
11043918
Knihovny.cz E-resources
- MeSH
- Antioxidants metabolism MeSH
- Erythrocytes enzymology MeSH
- Fibrinolytic Agents administration & dosage MeSH
- Glutathione Peroxidase metabolism MeSH
- Myocardial Infarction drug therapy metabolism MeSH
- Isoenzymes blood MeSH
- Creatine Kinase, MB Form MeSH
- Creatine Kinase blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Malondialdehyde blood MeSH
- Myocardial Reperfusion Injury metabolism MeSH
- Aged MeSH
- Streptokinase administration & dosage MeSH
- Superoxide Dismutase metabolism MeSH
- Thrombolytic Therapy MeSH
- Free Radicals metabolism MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Clinical Trial MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antioxidants MeSH
- Fibrinolytic Agents MeSH
- Glutathione Peroxidase MeSH
- Isoenzymes MeSH
- Creatine Kinase, MB Form MeSH
- Creatine Kinase MeSH
- Malondialdehyde MeSH
- Streptokinase MeSH
- Superoxide Dismutase MeSH
- Free Radicals MeSH
The study of ischemia/reperfusion injury included 25 patients in the acute phase of myocardial infarction (19 perfused, 6 remained non-reperfused as evaluated according to the time course of creatine kinase and CK-MB isoenzyme activity) and a control group (21 blood donors). Plasma level of malondialdehyde was followed as a marker of oxidative stress. Shortly after reperfusion (within 90 min), a transient increase of malondialdehyde concentration was detected. The return to the baseline level was achieved 6 h after the onset of therapy. The activity of a free radical scavenger enzyme, plasma glutathione peroxidase (GPx), reached its maximum 90 min after the onset of treatment and returned to the initial value after 18 h. The specificity of the GPx response was confirmed by comparing with both non-reperfused patients and the control group, where no significant increase was detected. The erythrocyte Cu,Zn-superoxide dismutase (SOD) did not exhibit significant changes during the interval studied in perfused patients, probably due to the stability of erythrocyte metabolism. In non-reperfused patients, a decrease of SOD was found during prolonged hypoxia. These results help to elucidate the mechanisms of fast activation of plasma antioxidant system during the reperfusion after myocardial infarction.