Poly[N-(2-hydroxypropyl)methacrylamide] polymers diffuse in brain extracellular space with same tortuosity as small molecules
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
NS 28642
NINDS NIH HHS - United States
PubMed
11159424
PubMed Central
PMC1301255
DOI
10.1016/s0006-3495(01)76036-5
PII: S0006-3495(01)76036-5
Knihovny.cz E-zdroje
- MeSH
- dextrany chemie farmakokinetika MeSH
- difuze MeSH
- extracelulární prostor metabolismus MeSH
- fluorescenční mikroskopie MeSH
- krysa rodu Rattus MeSH
- kyseliny polymethakrylové chemie farmakokinetika MeSH
- modely neurologické MeSH
- molekulová hmotnost MeSH
- neokortex metabolismus MeSH
- optika a fotonika MeSH
- potkani Sprague-Dawley MeSH
- sérový albumin hovězí chemie farmakokinetika MeSH
- skot MeSH
- systémy cílené aplikace léků MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- dextrany MeSH
- Duxon MeSH Prohlížeč
- kyseliny polymethakrylové MeSH
- sérový albumin hovězí MeSH
Integrative optical imaging was used to show that long-chain synthetic poly[N-(2-hydroxypropyl)methacrylamide] (PHPMA) polymers in a range of molecular weights from 7.8 to 1057 kDa were able to diffuse through the extracellular space in rat neocortical slices. Tortuosity (square root of ratio of diffusion coefficient in aqueous medium to that in brain) measured with such polymers averaged 1.57, a value similar to that obtained previously with tetramethylammonium, a small cation. When PHPMA was conjugated with bovine serum albumin (BSA) to make a bulky polymer with molecular weight 176 kDa, the tortuosity rose to 2.27, a value similar to that obtained previously with BSA alone and with 70-kDa dextran. The method of image analysis was justified with diffusion models involving spherical and nonspherical initial distributions of the molecules.
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Diffusion in brain extracellular space
