Effect of IFN-gamma receptor gene deletion on vaccinia virus virulence
Jazyk angličtina Země Rakousko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11315635
DOI
10.1007/s007050170172
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- Cercopithecus aethiops MeSH
- delece genu * MeSH
- DNA primery MeSH
- interferon gama fyziologie MeSH
- králíci MeSH
- lidé MeSH
- myši MeSH
- polymerázová řetězová reakce MeSH
- receptor interferonu gama MeSH
- receptory interferonů nedostatek genetika fyziologie MeSH
- vakcínie imunologie MeSH
- virulence MeSH
- virus vakcinie patogenita fyziologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA primery MeSH
- interferon gama MeSH
- receptory interferonů MeSH
Two vaccina virus (VV) strains, WR and Praha, were selected for a study undertaken to determine whether the virus-encoded interferon-gamma receptor (IFN-gamma R) plays any role in virus virulence. Both of the viruses expressed the B8R gene coding for IFN-gamma R in infected cell cultures. The nucleotide sequence of the Praha virus B8R gene was determined, and, when compared with the published sequence of the WR virus, it only displayed one silent nucleotide substitution. Mutants of the WR and Praha viruses with deleted B8R gene were constructed. In rabbits, skin lesions produced by the WR B8R-deleted mutants were smaller and tended to disappear earlier than those caused by wild-type WR virus. Similar results were obtained with both independently prepared WR B8R-deleted mutants. These data strongly suggested that the product of B8R gene did play a role in virus virulence. A similar comparison of the wild-type Praha virus and its mutant could not be done because of the very low virulence of the parental virus for rabbits.
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