The respiratory complex I in yeast: isolation of a gene NUO51 coding for the nucleotide-binding subunit of NADH:ubiquinone oxidoreductase from the obligately aerobic yeast Yarrowia lipolytica

. 2000 ; 45 (5) : 429-33.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid11357863

We have isolated a gene NUO51 coding for a homologue of the nucleotide-binding subunit of mitochondrial respiratory chain linked NADH:ubiquinone oxidoreductase from the obligately aerobic yeast Yarrowia lipolytica. DNA sequencing revealed a 1464 bp open reading frame encoding a protein with predicted molar mass of about 53.7 kDa. The sequence is highly conserved with its counterparts from filamentous fungi and represents the first yeast homologue of the NADH-binding subunit (51 kDa) of the respiratory complex 1. In addition, PFGE and Southern hybridization analysis indicate that NUO51 is a single copy gene in the genome of Y. lipolytica. The expression of NUO51 by Northern blot analysis was also examined.

Zobrazit více v PubMed

J Bioenerg Biomembr. 1993 Aug;25(4):325-9 PubMed

Methods Enzymol. 1991;194:169-82 PubMed

Chromosoma. 1997 Nov;106(6):380-90 PubMed

Yeast. 1994 Apr;10(4):475-9 PubMed

J Cell Sci. 1999 Jul;112 ( Pt 14):2347-54 PubMed

Folia Microbiol (Praha). 1999;44(1):19-24 PubMed

J Bacteriol. 1994 Sep;176(18):5622-30 PubMed

Biochim Biophys Acta. 1974 Oct 31;346(2):101-35 PubMed

FEBS Lett. 1995 Jun 26;367(2):107-11 PubMed

J Biol Chem. 1975 Jan 10;250(1):211-7 PubMed

Eur J Biochem. 1994 Mar 1;220(2):551-8 PubMed

Nucleic Acids Symp Ser. 1995;(34):23-4 PubMed

J Mol Biol. 1993 Sep 5;233(1):109-22 PubMed

J Biol Chem. 1974 Jun 25;249(12):3819-26 PubMed

Hum Mol Genet. 1998;7(10):1573-9 PubMed

Methods Enzymol. 1991;194:3-21 PubMed

Zobrazit více v PubMed

GENBANK
AF146339, AF197536

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace

Nahrávání dat ...