Ribosomal RNA genes from Prevotella bryantii: organization and heterogeneity
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
11501481
DOI
10.1007/bf02825889
Knihovny.cz E-zdroje
- MeSH
- fylogeneze MeSH
- genetická variace genetika MeSH
- geny rRNA * MeSH
- klonování DNA MeSH
- mezerníky ribozomální DNA genetika MeSH
- molekulární sekvence - údaje MeSH
- Prevotella genetika MeSH
- RNA ribozomální 16S genetika MeSH
- RNA ribozomální 23S genetika MeSH
- RNA transferová specifická pro aminokyseliny genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mezerníky ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
- RNA ribozomální 23S MeSH
- RNA transferová specifická pro aminokyseliny MeSH
Five P. bryantii B(1)4 16S rRNA gene copies and their flanking regions were cloned and analyzed. A genomic library was constructed and screened with oligonucleotide DNA probe specific for 16S rRNA gene of P. bryantii. Five out of six different copies of 16S RNA gene were recovered and sequenced. Only minor differences (0.3-1.2%) between copies were detected within the 1541 bp long sequence. The impact of the sequence variability of 16S rRNA gene copies on phylogenetic positioning of P. bryantii was determined. All five sequences from cloned P. bryantii B(1)4 16S rRNA genes were placed in the same operational taxonomy unit. Control regions of all five analyzed rRNA operons were almost identical and three candidate for promoter sequences were identified by Neutral Network Promoter Prediction. Spacer regions between 16S rRNA and 23S rRNA genes in all five cloned copies were 543 bp long and genes for tRNA(Ile) and tRNA(Ala) were identified inside this regions.
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