Ribosomal RNA genes from Prevotella bryantii: organization and heterogeneity
Language English Country United States Media print
Document type Journal Article
PubMed
11501481
DOI
10.1007/bf02825889
Knihovny.cz E-resources
- MeSH
- Phylogeny MeSH
- Genetic Variation genetics MeSH
- Genes, rRNA * MeSH
- Cloning, Molecular MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Molecular Sequence Data MeSH
- Prevotella genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- RNA, Ribosomal, 23S genetics MeSH
- RNA, Transfer, Amino Acid-Specific genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Ribosomal Spacer MeSH
- RNA, Ribosomal, 16S MeSH
- RNA, Ribosomal, 23S MeSH
- RNA, Transfer, Amino Acid-Specific MeSH
Five P. bryantii B(1)4 16S rRNA gene copies and their flanking regions were cloned and analyzed. A genomic library was constructed and screened with oligonucleotide DNA probe specific for 16S rRNA gene of P. bryantii. Five out of six different copies of 16S RNA gene were recovered and sequenced. Only minor differences (0.3-1.2%) between copies were detected within the 1541 bp long sequence. The impact of the sequence variability of 16S rRNA gene copies on phylogenetic positioning of P. bryantii was determined. All five sequences from cloned P. bryantii B(1)4 16S rRNA genes were placed in the same operational taxonomy unit. Control regions of all five analyzed rRNA operons were almost identical and three candidate for promoter sequences were identified by Neutral Network Promoter Prediction. Spacer regions between 16S rRNA and 23S rRNA genes in all five cloned copies were 543 bp long and genes for tRNA(Ile) and tRNA(Ala) were identified inside this regions.
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Folia Microbiol (Praha). 2001;46(1):40-4 PubMed
Methods Enzymol. 1987;152:1-812 PubMed
FEMS Microbiol Ecol. 2000 Jul 1;33(1):69-79 PubMed
J Bacteriol. 1995 Oct;177(20):5884-90 PubMed
J Bacteriol. 1990 Jul;172(7):3620-30 PubMed
J Bacteriol. 1991 Nov;173(21):6919-26 PubMed
FEMS Microbiol Lett. 1995 Jan 15;125(2-3):135-41 PubMed
Int J Syst Bacteriol. 1994 Apr;44(2):246-55 PubMed
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 PubMed
Int J Syst Bacteriol. 1997 Apr;47(2):284-8 PubMed
Am J Clin Nutr. 1972 Dec;25(12):1324-8 PubMed
Microbiol Rev. 1995 Mar;59(1):143-69 PubMed
Comput Appl Biosci. 1996 Aug;12(4):357-8 PubMed
J Bacteriol. 1997 May;179(10):3270-6 PubMed
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