Restriction and modification systems of ruminal bacteria
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11501482
DOI
10.1007/bf02825890
Knihovny.cz E-resources
- MeSH
- Rumen microbiology MeSH
- DNA Restriction-Modification Enzymes classification metabolism MeSH
- Gram-Negative Anaerobic Straight, Curved, and Helical Rods enzymology MeSH
- Selenomonas classification enzymology MeSH
- Streptococcus bovis enzymology MeSH
- Deer MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Restriction-Modification Enzymes MeSH
A high frequency of type II restriction endonuclease activities was detected in Selenomonas ruminantium but not in other rumen bacteria tested. Eight different restriction endonucleases were characterized in 17 strains coming from genetically homogeneous local population. Chromosomal DNA isolated from S. ruminantium strains was found to be refractory to cleavage by various restriction enzymes, implying the presence of methylase activities additional to those required for protection against the cellular endonucleases. The presence of Dam methylation was detected in S. ruminantium strains as well as in several other species belonging to the Sporomusa subbranch of low G + C Gram-positive bacteria (Megasphaera elsdenii, Mitsuokella multiacidus).
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