Changes in microtubules and microfilaments due to a combined effect of ultrasound and cytostatics in HeLa cells
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11508858
Knihovny.cz E-resources
- MeSH
- Biopolymers MeSH
- HeLa Cells drug effects ultrastructure MeSH
- Humans MeSH
- Methotrexate pharmacology toxicity MeSH
- Actin Cytoskeleton drug effects ultrastructure MeSH
- Microtubules drug effects ultrastructure MeSH
- Organoplatinum Compounds pharmacology toxicity MeSH
- Antineoplastic Agents pharmacology MeSH
- Ultrasonics * adverse effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biopolymers MeSH
- cycloplatam MeSH Browser
- Methotrexate MeSH
- Organoplatinum Compounds MeSH
- Antineoplastic Agents MeSH
Treatment of HeLa cells with low intensity ultrasound and two cytostatic drugs, cycloplatin and methotrexate, resulted in a partial disassembly of microtubules and microfilaments. This disassembly was due to depolymerization and subsequent erroneous repolymeration of essential cytoskeletal proteins, resulting in formation of unusual arrangements, mainly small, granule-like structures. The combined action of ultrasound and cytostatics had a synergistic effect dependent on both the concentration of the drug and the time of sonication. The demonstrated changes in the cytoskeleton are considered to be non-specific to ultrasound treatment, reflecting only an altered vital state of the treated cells.
