Cryopreservation of basidiomycete strains using perlite
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11714521
DOI
10.1016/s0167-7012(01)00338-4
PII: S0167701201003384
Knihovny.cz E-resources
- MeSH
- Basidiomycota growth & development MeSH
- Time Factors MeSH
- Cryopreservation methods MeSH
- Cryoprotective Agents MeSH
- Aluminum Oxide * MeSH
- Silicon Dioxide * MeSH
- Freezing MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cryoprotective Agents MeSH
- Aluminum Oxide * MeSH
- Silicon Dioxide * MeSH
- Perlite MeSH Browser
A new alternative method using perlite as a particulate solid carrier in the growth medium with a cryoprotectant was successfully tested for cryopreservation of several basidiomycete species from different genera (Armillaria, Pleurotus, Pluteus, Polyporus) which failed to survive or retain their properties in cryopreservation procedures routinely used in our laboratory. Frozen basidiomycete strains were kept in cryovials submerged in liquid nitrogen and were either immediately after the freezing process or after a 6-month storage thawed and checked for viability, purity and changes in growth, morphology and biochemical characteristics. All cultures survived the cryopreservation procedure and no negative effects of cryopreservation by this method have been observed after 6 months of storage in liquid nitrogen.
References provided by Crossref.org
Millipede gut-derived microbes as a potential source of cellulolytic enzymes
Long-term maintenance of fungal cultures on perlite in cryovials - an alternative for agar slants
Cryopreservation of filamentous micromycetes and yeasts using perlite
