A comparison of whole blood neutrophil chemiluminescence measured with cuvette and microtitre plate luminometers
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
11816055
DOI
10.1002/bio.664
PII: 10.1002/bio.664
Knihovny.cz E-resources
- MeSH
- Neutrophil Activation drug effects MeSH
- Calcimycin pharmacology MeSH
- Fluorometry instrumentation standards MeSH
- Physics instrumentation MeSH
- Kinetics MeSH
- Blood Cells chemistry immunology metabolism MeSH
- Humans MeSH
- Luminescent Measurements MeSH
- Methods MeSH
- N-Formylmethionine Leucyl-Phenylalanine pharmacology MeSH
- Neutrophils chemistry immunology metabolism MeSH
- Specimen Handling methods MeSH
- Tetradecanoylphorbol Acetate pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Calcimycin MeSH
- N-Formylmethionine Leucyl-Phenylalanine MeSH
- Tetradecanoylphorbol Acetate MeSH
Results obtained by measuring human whole blood neutrophil chemiluminescence (CL) using the BioOrbit 1251 cuvette luminometer and the Immunotech LM-01T microtitre plate luminometer are compared in this study. Opsonized zymosan, phorbol myristate acetate, N-formyl-Met-Leu-Phe and calcium ionophore A23187 were used as activators. The CL response of neutrophils to their stimulation with the individual types of activators tested was fully detectable using either type of the luminometers. The kinetic curves of CL activity obtained from both the cuvette and the microtitre plate luminometers had similar characteristics. The only insignificant difference observed when comparing the kinetic curves was in the rates of the CL reactions. The peak CL response of activated neutrophils was reached faster when using the luminometer BioOrbit 1251 than with the luminometer Immunotech LM-01T. A likely reason for this difference is the mode of transporting samples during the measurement, inducing different degrees of agitation. However, although this fact needs to be considered when interpreting results, both types of luminometer can be fully utilized in both research and clinical laboratories.
References provided by Crossref.org
Flavonoids inhibit the respiratory burst of neutrophils in mammals
