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Bifidobacterium bifidum monoassociation of gnotobiotic mice: effect on enterocyte brush-border enzymes

Kozáková, H
Author Kozáková, H Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 549 22 Nový Hrádek, Czechia. kozakova@biomed.cas.cz
Reháková, Z
Author Reháková, Z
Kolínská, J
Author Kolínská, J

Folia microbiologica. 2001 ; 46 (6) : 573-6.

Folia Microbiol (Praha)
ISSN 0015-5632
Source

Language English Country United States Media print

Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid11898351

PubMed 11898351
DOI 10.1007/bf02818005
Knihovny.cz E-resources

MeSH
Alkaline Phosphatase analysis MeSH
beta-Galactosidase analysis MeSH
Bifidobacterium * MeSH
gamma-Glutamyltransferase analysis MeSH
Glucan 1,4-alpha-Glucosidase analysis MeSH
Germ-Free Life physiology MeSH
Lactase MeSH
Sucrase analysis MeSH
Intestines enzymology microbiology MeSH
Animals MeSH
Check Tag
Male MeSH
Female MeSH
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Comparative Study MeSH
Names of Substances
Alkaline Phosphatase MeSH
beta-Galactosidase MeSH
gamma-Glutamyltransferase MeSH
Glucan 1,4-alpha-Glucosidase MeSH
Lactase MeSH
Sucrase MeSH

The effect of intestinal colonization with Bifidobacterium bifidum (Gram-positive anaerobic bacterium colonizing the intestine of healthy new-born mammals, exhibiting a probiotic effect, protecting the intestinal mucosa against colonization by pathogenic microflora) on enterocyte brush-border enzymes was examined in weaned 23-d- and in 2-month-old gnotobiotic inbred mice and compared with that in corresponding germ-free (GF) and conventional (CV) controls. The two groups of GF mice were associated with human B. bifidum 11 d before the end of the experiment. Specific activity of enterocyte brush-border enzymes--lactase, alkaline phosphatase and gamma-glutamyltranspeptidase was significantly higher in both age groups of GF mice in comparison with CV ones; on the other hand, sucrase and glucoamylase activities were higher in CV mice. Monoassociation with B. bifidum accelerates biochemical maturation of enterocytes resulting in a shift of specific activities of brush-border enzymes between the values found for GF and CV mice. This effect of B. bifidum supplementation was less pronounced for alkaline phosphatase, sucrase, glucoamylase and dipeptidyl peptidase i.v. in immature gut of weaned mice than of 2-month-old ones.

Department of Immunology and Gnotobiology Institute of Microbiology Academy of Sciences of the Czech Republic 549 22 Nový Hrádek Czechia

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