Comparison of antagonistic ability against enteropathogens by G+ and G- anaerobic dominant components of human fecal microbiota
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16821725
DOI
10.1007/bf02932170
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky biosyntéza MeSH
- bakteriologické techniky metody MeSH
- dospělí MeSH
- ekosystém MeSH
- feces mikrobiologie MeSH
- gramnegativní anaerobní bakterie izolace a purifikace metabolismus MeSH
- inhibitory růstu biosyntéza izolace a purifikace MeSH
- lidé MeSH
- Salmonella typhimurium růst a vývoj MeSH
- Shigella sonnei růst a vývoj MeSH
- střeva mikrobiologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- inhibitory růstu MeSH
To confirm if anaerobic G+-components are those responsible for the function of colonization resistance, obligate anaerobic G+- and G- -bacteria from normal dominant microbiota of human feces were isolated from three successive collections and then used in in vitro assays for antagonism against two enteropathogenic bacteria. The production of inhibitory diffusible compounds was determined on supplemented BHI agar and MRS agar media for G- - and G+-bacteria, respectively. Salmonella enterica subsp. enterica serovar Typhimurium and Shigella sonnei were used as indicators. G+-bacteria presented a higher overall antagonistic frequency against both pathogenic bacteria (57 and 64 % for S. enterica serovar Typhimurium and S. sonnei, respectively) when compared to G+-microorganisms but with a quite elevated variation between volunteers (0-100 %) and collection samples (40-72 and 40-80 % for S. enterica sv. Typhimurium and S. sonnei, respectively). On the other hand, only three among 143 G- -isolates tested showed antagonistic activity. The results showed that, at least in vitro, obligate anaerobic G+-components of the dominant human fecal microbiota present a higher potential for antagonism against the enteropathogenic models tested than do G- -bacteria.
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