Subcellular shifts of trimeric G-proteins following activation of baker's yeast by glucose
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11899470
DOI
10.1007/bf02814427
Knihovny.cz E-zdroje
- MeSH
- glukosa farmakologie MeSH
- imunoblotting MeSH
- proteiny vázající GTP analýza biosyntéza MeSH
- Saccharomyces cerevisiae účinky léků metabolismus MeSH
- subcelulární frakce metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- glukosa MeSH
- proteiny vázající GTP MeSH
Addition of glucose to a resting cell suspension of the yeast Saccharomyces cerevisiae was accompanied by marked shifts of the G alpha-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between < 10 and 20 s. The decrease of the plasma membrane pool of the Gi alpha/Go alpha- and Gq alpha/Gl 1 alpha-protein subunits correlated with an increase in acid-sensitive forms of these proteins which was recovered in the mitochondrial and/or lysosomal membrane fraction. In contrast to cells from higher organisms glucose-stimulated yeast exhibits an extremely rapid type of the redistribution (internalization). The question remains open as to the functional significance of the internalized forms of the G-proteins as these remain sequestered from the plasma membrane well after glucose has been consumed.
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Arch Physiol Biochem. 1995 May;103(2):202-10 PubMed
J Biol Chem. 1995 Jan 13;270(2):503-6 PubMed
J Biol Chem. 1999 Feb 26;274(9):5843-50 PubMed
J Biol Chem. 1992 Nov 15;267(32):23409-17 PubMed
Proc Natl Acad Sci U S A. 1990 Jan;87(2):568-72 PubMed
J Biol Chem. 1998 Aug 21;273(34):21699-707 PubMed
J Mol Cell Cardiol. 1999 Apr;31(4):761-72 PubMed
FEBS Lett. 1983 May 30;156(1):11-4 PubMed
Cell Biol Int. 1994 Aug;18(8):813-7 PubMed
Proc Natl Acad Sci U S A. 1989 Oct;86(20):7900-3 PubMed
EMBO J. 1998 Apr 1;17(7):1996-2007 PubMed
Biochem J. 1995 Sep 15;310 ( Pt 3):1021-7 PubMed
J Gen Microbiol. 1992 Aug;138 Pt 8:1579-86 PubMed
Eur J Biochem. 1994 Sep 1;224(2):455-62 PubMed
Semin Cell Dev Biol. 1998 Apr;9(2):135-41 PubMed
Dev Biol. 1994 Dec;166(2):363-79 PubMed
EMBO J. 1998 Jun 15;17(12):3326-41 PubMed
Biochemistry. 1992 Sep 1;31(34):8030-5 PubMed
Trends Biochem Sci. 1991 Sep;16(9):338-41 PubMed
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3675-9 PubMed
Proc Natl Acad Sci U S A. 1990 Jan;87(2):728-32 PubMed
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2800-4 PubMed
FEBS Lett. 1985 Apr 8;183(1):21-4 PubMed
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14592-7 PubMed
Biochim Biophys Acta. 1981 May 20;643(3):572-82 PubMed
Mol Pharmacol. 1996 Apr;49(4):646-55 PubMed
J Biol Chem. 1994 Apr 8;269(14):10393-9 PubMed
