Muscovite (mica) allows the characterisation of supported bilayers by ellipsometry and confocal fluorescence correlation spectroscopy
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11934273
DOI
10.1515/bc.2002.037
Knihovny.cz E-resources
- MeSH
- Microscopy, Fluorescence MeSH
- Phosphatidylcholines chemistry MeSH
- Phosphatidylserines chemistry MeSH
- Phospholipids chemistry MeSH
- Membrane Fusion MeSH
- Microscopy, Confocal MeSH
- Lipid Bilayers chemistry MeSH
- Surface Properties MeSH
- Aluminum Silicates chemistry MeSH
- Silicates chemistry MeSH
- Boron Compounds chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2-dioleoylphosphatidylserine MeSH Browser
- 1,2-oleoylphosphatidylcholine MeSH Browser
- Phosphatidylcholines MeSH
- Phosphatidylserines MeSH
- Phospholipids MeSH
- Lipid Bilayers MeSH
- mica MeSH Browser
- Aluminum Silicates MeSH
- Silicates MeSH
- Boron Compounds MeSH
We demonstrate for the first time that ellipsometry and confocal fluorescence correlation spectroscopy (FCS) are complementary methods for the characterisation of supported planar phospholipid bilayers (SPBs) formed on mica, a mineral used in atomic force microscopy investigations of SPBs. Addition of small unilamellar vesicles containing 20% dioleoyl-phosphatidylserine (DOPS) and 80% dioleoyl-phosphatidylcholine (DOPC) to an oxidised borosilicate surface, on the other hand, results in a planar lipid system characterised by lateral diffusion coefficients which are three time smaller than those obtained for SPBs. Moreover, seven labelled phospholipids were tested for their suitability in the FCS characterisation of vesicles as well as of SPBs.
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