Muscovite (mica) allows the characterisation of supported bilayers by ellipsometry and confocal fluorescence correlation spectroscopy
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11934273
DOI
10.1515/bc.2002.037
Knihovny.cz E-zdroje
- MeSH
- fluorescenční mikroskopie MeSH
- fosfatidylcholiny chemie MeSH
- fosfatidylseriny chemie MeSH
- fosfolipidy chemie MeSH
- fúze membrán MeSH
- konfokální mikroskopie MeSH
- lipidové dvojvrstvy chemie MeSH
- povrchové vlastnosti MeSH
- silikáty hliníku chemie MeSH
- silikáty chemie MeSH
- sloučeniny boru chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,2-dioleoylphosphatidylserine MeSH Prohlížeč
- 1,2-oleoylphosphatidylcholine MeSH Prohlížeč
- fosfatidylcholiny MeSH
- fosfatidylseriny MeSH
- fosfolipidy MeSH
- lipidové dvojvrstvy MeSH
- mica MeSH Prohlížeč
- silikáty hliníku MeSH
- silikáty MeSH
- sloučeniny boru MeSH
We demonstrate for the first time that ellipsometry and confocal fluorescence correlation spectroscopy (FCS) are complementary methods for the characterisation of supported planar phospholipid bilayers (SPBs) formed on mica, a mineral used in atomic force microscopy investigations of SPBs. Addition of small unilamellar vesicles containing 20% dioleoyl-phosphatidylserine (DOPS) and 80% dioleoyl-phosphatidylcholine (DOPC) to an oxidised borosilicate surface, on the other hand, results in a planar lipid system characterised by lateral diffusion coefficients which are three time smaller than those obtained for SPBs. Moreover, seven labelled phospholipids were tested for their suitability in the FCS characterisation of vesicles as well as of SPBs.
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