Alloantigen-induced, T-cell-dependent production of nitric oxide by macrophages infiltrating skin allografts in mice
Jazyk angličtina Země Švýcarsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- časové faktory MeSH
- DNA primery MeSH
- homologní transplantace imunologie MeSH
- imunosupresivní léčba metody MeSH
- isoantigeny imunologie MeSH
- kinetika MeSH
- kokultivační techniky MeSH
- lymfatické uzliny cytologie MeSH
- lymfocytární deplece MeSH
- makrofágy cytologie imunologie MeSH
- myši inbrední A MeSH
- myši inbrední BALB C MeSH
- myši inbrední C57BL MeSH
- myši inbrední DBA MeSH
- myši MeSH
- oxid dusnatý biosyntéza MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- sekvence nukleotidů MeSH
- synthasa oxidu dusnatého, typ II MeSH
- synthasa oxidu dusnatého genetika MeSH
- T-lymfocyty imunologie MeSH
- transplantace izogenní imunologie MeSH
- transplantace kůže imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA primery MeSH
- isoantigeny MeSH
- Nos2 protein, mouse MeSH Prohlížeč
- oxid dusnatý MeSH
- synthasa oxidu dusnatého, typ II MeSH
- synthasa oxidu dusnatého MeSH
The immunological rejection reaction occurring after organ or tissue transplantation is characterized by a strong infiltration of the graft by T cells and macrophages. Since the rejection reaction is highly specific, we tested the role of T cells in the activation of macrophages and in the induction of nitric oxide (NO) production during graft rejection. The rejection of both MHC and non-MHC antigen-disparate skin allografts was associated with a significantly increased production of NO in the graft. The kinetics of NO production after transplantation correlated with the rejection reaction and with the fate of the allograft. A significant reduction in NO production was found in immunologically hyporeactive mice treated with cyclosporine, and no specific production of NO was found in tolerated skin allografts from neonatally tolerant mice. The production of NO was completely suppressed in graft explants from mice with depleted CD4(+) cells, but remained at a normal level in skin allografts from mice treated with anti-CD8 monoclonal antibody. The treatment of recipients of fully allogeneic skin grafts with 2-amino-5,6-dihydro-6-methyl-4H-1,3-thiazine (AMT), a specific inhibitor of the inducible NO synthase, resulted in a significant prolongation of graft survival. The results thus show CD4(+) T-cell-dependent, alloantigen-induced production of NO by graft-infiltrating macrophages and the role of NO in the rejection reaction. We suggest that this pathway may represent one of the local effector mechanisms of graft rejection.
Citace poskytuje Crossref.org
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