Oriented immobilization of galactose oxidase to bead and magnetic bead cellulose and poly(HEMA-co-EDMA) and magnetic poly(HEMA-co-EDMA) microspheres
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12013233
DOI
10.1016/s0378-4347(01)00439-x
PII: S037843470100439X
Knihovny.cz E-zdroje
- MeSH
- celulosa chemie MeSH
- enzymy imobilizované chemie MeSH
- galaktosaoxidasa chemie MeSH
- imunoglobulin G chemie MeSH
- kyselina jodistá chemie MeSH
- magnetismus MeSH
- methakryláty chemie MeSH
- mikrosféry MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- polyhydroxyethylmethakrylát chemie MeSH
- prasata MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- celulosa MeSH
- enzymy imobilizované MeSH
- ethylene dimethacrylate MeSH Prohlížeč
- galaktosaoxidasa MeSH
- imunoglobulin G MeSH
- kyselina jodistá MeSH
- metaperiodate MeSH Prohlížeč
- methakryláty MeSH
- polyhydroxyethylmethakrylát MeSH
In order to obtain an active and stable oxidation reactor for daily use in biochemical laboratory we decided to immobilize galactose oxidase orientedly through a carbohydrate chain to the magnetic carriers. We used hydrazide derivatives of non-magnetic and magnetic bead cellulose and of magnetic and non-magnetic poly(HEMA-co-EDMA) microspheres. Activation of the enzyme molecules was done by sodium periodate in the presence of supplements (fucose, CuSO4, catalase). Orientedly immobilized galactose oxidase presents high storage stability and lower susceptibility to inappropriate microenvironmental conditions. Reactor reactivated by three pulses of D-galactose retained practically 100% of its native activity after 6 months. The positive properties of both magnetic carriers were entirely confirmed.
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