Specimens of the Paradiplozoon homoion-complex were collected from ten species of cyprinid fish in the Czech Republic. A combined molecular and morphometric approach was performed to distinguish Paradiplozoon homoion and P. gracile. The second internal transcribed spacer (ITS2) of the ribosomal RNA genes was amplified and sequences were analysed. No variability in the analysed sequences was detected. Measurements of clamps and the central hooks obtained from specimens from different host species were compared. Great variability was found in the length and width of the third pair of clamps. No significant differences were detected in the measurements of the central hook sickle. A positive relationship was found between host size and each of the following measurements of the third pair clamps: length and width of the whole clamp; and length of the median plate of the third pair of clamps. The length of the median plate of the attachment clamps may be a useful character for species identification of diplozoids. Further molecular and morphometric studies are required to resolve this taxonomic problem and, henceforth, we suggest considering P. gracile as a species inquirenda.

Department of Zoology and Ecology Faculty of Science Masaryk University Kotlarská 2 61137 Brno Czech Republic

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Syst Parasitol. 2001 Feb;48(2):141-50 PubMed

Ann Parasitol Hum Comp. 1973 Mar-Jun;48(3):447-56 PubMed

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Int J Parasitol. 1991 Feb;21(1):113-4 PubMed

Int J Parasitol. 1992 May;22(3):403-6 PubMed

Parassitologia. 1997 Sep;39(3):189-99 PubMed

Dis Aquat Organ. 2001 Aug 2;45(3):191-6 PubMed

Z Parasitenkd. 1964 Nov 17;25:164-92 PubMed

Int J Parasitol. 2001 May 15;31(7):738-45 PubMed

C R Acad Sci III. 1996 Feb;319(2):113-8 PubMed

J Parasitol. 1997 Apr;83(2):215-9 PubMed

Folia Parasitol (Praha). 1976;23(2):111-26 PubMed

Mol Phylogenet Evol. 2000 Feb;14(2):165-79 PubMed

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