Paradiplozoon hemiculteri (Ling, 1973), a member of the Diplozoidae, parasitizes the gills of Asian fish. Not only is the type material unavailable for this species, the original description was poor and somewhat conflicting, and adequate molecular data were not available. What is more, the available morphological and molecular data are inconsistent and fluctuate significantly. Here, we present a redescription of P. hemiculteri based on morphological and molecular data from new isolates collected from the type host, the sharpbelly Hemiculter leucisculus (Basilewsky, 1855), captured at the neotype locality (Shaoguan, Guangdong Province, southern China); a neotype for P. hemiculteri was designated from this collection. The length and width of the body, buccal suckers, pharynx, attachment clamps, sickle and the central hook handle were all measured and the shape of the anterior and posterior part of the median plate and anterior and posterior joining sclerites accurately documented. Phylogenetic analyses based on the sequences of the second rDNA internal transcribed spacer (ITS2) indicated that all new samples clustered together and differed clearly from sequences attributed to P. hemiculteri, which are deposited in GenBank. Our results confirm that P. hemiculteri is the only diplozoid that has demonstrably been found on the gills of H. leucisculus to date.

Paradiplozoon hemiculteri (Ling, 1973), membre des Diplozoidae, parasite les branchies des poissons asiatiques. Non seulement le matériel-type n’est pas disponible pour cette espèce, mais la description originale était médiocre et quelque peu contradictoire, et des données moléculaires adéquates n’étaient pas disponibles. Qui plus est, les données morphologiques et moléculaires disponibles sont incohérentes et fluctuent de manière significative. Ici, nous présentons une nouvelle description de P. hemiculteri basée sur des données morphologiques et moléculaires provenant de nouveaux isolats recueillis chez l’hôte-type, Hemiculter leucisculus (Basilewsky, 1855), capturé à la localité du néotype (Shaoguan, province du Guangdong, sud de la Chine); un néotype pour P. hemiculteri a été désigné à partir de cette collection. La longueur et la largeur du corps, les ventouses buccales, le pharynx, les pinces, la faucille et la poignée centrale ont été mesurés et la forme de la partie antérieure et postérieure de la plaque médiane et des sclérites antérieurs et postérieurs bien documentée. Des analyses phylogénétiques basées sur les séquences du second espaceur transcrit interne de l’ADNr (ITS2) ont indiqué que tous les nouveaux échantillons étaient regroupés et différaient clairement des séquences attribuées à P. hemiculteri qui sont déposées dans GenBank. Nos résultats confirment que P. hemiculteri est le seul Diplozoidae qui ait été trouvé sur les branchies de H. leucisculus à ce jour.

Department of Botany and Zoology Faculty of Science Masaryk University Kotlářská 2 611 37 Brno Czech Republic

Department of Botany and Zoology Faculty of Science Masaryk University Kotlářská 2 611 37 Brno Czech Republic Department of Forest Botany Dendrology and Geobiocoenology Faculty of Forestry and Wood Technology Mendel University in Brno Zemědělská 3 613 00 Brno Czech Republic

Department of Botany and Zoology Faculty of Science Masaryk University Kotlářská 2 611 37 Brno Czech Republic Department of Parasitology Faculty of Science Charles University Viničná 7 128 44 Prague Czech Republic

School of Life Science South China Normal University Guangzhou 510631 People's Republic of China

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