Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
12417010
Knihovny.cz E-zdroje
- MeSH
- buněčná membrána fyziologie MeSH
- draslík fyziologie MeSH
- ionty MeSH
- kultivované buňky MeSH
- motilita spermií fyziologie MeSH
- osmolární koncentrace MeSH
- ryby fyziologie MeSH
- vápník fyziologie MeSH
- videomikroskopie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Názvy látek
- draslík MeSH
- ionty MeSH
- vápník MeSH
This study investigated the effects of different environmental conditions on the motility parameters of paddlefish (Polyodon spathula) spermatozoa. Paddlefish spermatozoa demonstrated the following characteristics: (i) all spermatozoa were motile 10 s after activation with a velocity of 130-160 microm s(-1); (ii) after 2 min, velocity decreased to 80-130 microm s(-1); and (iii) motility was maintained for up to 9 min. Concentrations of 0.5-5.0 mmol KCl l(-1) prevented activation of spermatozoa. After transfer into a swimming medium (20 mmol Tris l(-1), pH 8.2 and 1 mg BSA ml(-1)) containing 0.5 mmol KCl l(-1) (combined with 5 mmol NaCl or MgCl(2) l(-1)), 80-100% of cells were motile with a velocity of about 120-150 microm s(-1). MgCl(2) significantly improved the velocity of spermatozoa at 10, 40, 50 and 60 s after activation and the stable velocity of spermatozoa was about 140 microm s(-1). Very low concentrations of CaCl(2) (0.125 mmol l(-1)) combined with 0.5 mmol KCl l(-1) initiated motility in 20% of spermatozoa, whereas all spermatozoa were activated after 2 min with 0.25 mmol CaCl(2) l(-1) in similar medium for the full period of swimming with velocity of about 120 microm s(-1). This study demonstrated that potassium (5-15 mmol l(-1)) inhibits demembranated spermatozoa. Thus, initiation of movement in paddlefish spermatozoa is under the reciprocal control of potassium and calcium ion concentrations.
