Some features of DNA-binding proteins involved in the regulation of the Streptomyces aureofaciens gap gene, encoding glyceraldehyde-3-phosphate dehydrogenase
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12422508
DOI
10.1007/bf02818688
Knihovny.cz E-zdroje
- MeSH
- bakteriální geny * MeSH
- bakteriální proteiny genetika metabolismus MeSH
- DNA bakterií genetika MeSH
- DNA vazebné proteiny genetika metabolismus MeSH
- Escherichia coli genetika MeSH
- glyceraldehyd-3-fosfátdehydrogenasy genetika MeSH
- molekulární sekvence - údaje MeSH
- promotorové oblasti (genetika) MeSH
- regulace genové exprese enzymů MeSH
- regulace genové exprese u bakterií MeSH
- rekombinantní proteiny genetika metabolismus MeSH
- restrikční mapování MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- Streptomyces aureofaciens genetika růst a vývoj metabolismus MeSH
- trans-aktivátory genetika metabolismus MeSH
- vazebná místa genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- DNA vazebné proteiny MeSH
- GapR protein, Streptomyces aureofaciens MeSH Prohlížeč
- glyceraldehyd-3-fosfátdehydrogenasy MeSH
- rekombinantní proteiny MeSH
- trans-aktivátory MeSH
A gapR gene, encoding a protein similar to the AraC/XylS family of bacterial transcriptional regulators, was previously identified upstream of the gap gene, coding for glyceraldehyde-3-phosphate dehydrogenase in Streptomyces aureofaciens. The GapR protein overproduced in Escherichia coli was shown to bind to the gap-P promoter region. Using the gel mobility shift assay with cell-free protein extracts from different developmental stages of S. aureofaciens, we identified several other proteins, in addition to GapR, that specifically bound to the S. aureofaciens gap-P promoter region. When cell-free extracts from S. aureofaciens cultivated in liquid medium with glucose were analyzed, only one complex corresponding to GapR was detected. A new protein interacting with the gap-P promoter was detected in stationary culture of S. aureofaciens grown in the presence of mannitol as carbon sources. The GapR protein was partially purified from S. aureofaciens cultivated in liquid medium containing glucose and used for binding studies. DNA footprinting analysis revealed an identical protected region as previously identified for the GapR protein overproduced from Escherichia coli. The direct role of the GapR protein in the regulation of gap expression in S. aureofaciens in vivo was confirmed but regulation of gap expression seems to be more complex, possibly involving other regulatory protein(s), depending on the developmental stage of S. aureofaciens.
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Anal Biochem. 1976 May 7;72:248-54 PubMed
J Biol Chem. 1997 Jun 13;272(24):15106-12 PubMed
Methods Enzymol. 1980;65(1):499-560 PubMed
Folia Microbiol (Praha). 2001;46(6):527-34 PubMed
Eur J Biochem. 1989 Feb 1;179(2):405-13 PubMed
Gene. 1989 Jan 30;75(1):145-55 PubMed
J Biol Chem. 2000 May 12;275(19):14031-7 PubMed
Microbiology (Reading). 1998 Jun;144(6):1465-1478 PubMed
J Bacteriol. 1999 Nov;181(22):6996-7004 PubMed
J Bacteriol. 1988 Apr;170(4):1926-33 PubMed
J Bacteriol. 1983 Sep;155(3):1238-48 PubMed
J Bacteriol. 1994 Feb;176(3):830-9 PubMed
Mol Microbiol. 1989 Jun;3(6):723-32 PubMed
Microbiol Mol Biol Rev. 1997 Dec;61(4):393-410 PubMed
Microbiology (Reading). 2001 May;147(Pt 5):1291-1301 PubMed
Microbiology (Reading). 1998 Apr;144 ( Pt 4):905-914 PubMed
Anal Biochem. 2001 Jun 1;293(1):138-9 PubMed
J Bacteriol. 1998 Dec;180(24):6476-83 PubMed
Microbiology (Reading). 1997 Nov;143 ( Pt 11):3555-3561 PubMed
Folia Microbiol (Praha). 1998;43(6):605-12 PubMed
J Bacteriol. 1990 Aug;172(8):4329-38 PubMed
Gene. 1995 Nov 7;165(1):77-80 PubMed
