Identification of DNA-binding proteins involved in regulation of expression of the Streptomyces aureofaciens whiH gene encoding a sporulation transcription factor
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11898343
DOI
10.1007/bf02817997
Knihovny.cz E-resources
- MeSH
- Bacterial Proteins analysis metabolism MeSH
- DNA Footprinting MeSH
- DNA-Binding Proteins analysis metabolism MeSH
- Molecular Sequence Data MeSH
- Promoter Regions, Genetic MeSH
- Gene Expression Regulation, Bacterial * MeSH
- Repressor Proteins genetics MeSH
- Electrophoretic Mobility Shift Assay MeSH
- Base Sequence MeSH
- Streptomyces chemistry genetics growth & development MeSH
- Transcription Factors genetics MeSH
- Binding Sites MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- DNA-Binding Proteins MeSH
- Repressor Proteins MeSH
- Transcription Factors MeSH
- whiH protein, Streptomyces MeSH Browser
Using the gel mobility-shift assay with protein fractions from different developmental stages of solid-grown Streptomyces aureofaciens, we identified two different proteins specifically bound to the whiH promoter region. Only one protein (RwhA) was detected in young substrate mycelium cultivated in liquid medium. On comparing the mobility of the resulting complexes, one of the bound proteins present in substrate mycelium and in early stages of aerial mycelium seemed to be identical with the RwhA. The other detected protein with a higher mobility (RwhB) was present in all developmental stages except for mature spores. DNA footprinting analysis localized the binding site of RwhB to nucleotides -23 to +40 relative to the transcription start point of the PwhiH promoter. RwhA from young substrate mycelium protected the DNA fragment from -106 to -77 in coding strand and -126 to -82 in noncoding strand. WhiH has homology to a large family of metabolism-related repressors and seems to regulate negatively its own expression. These observations (and the results of transcription analysis of the whiH gene obtained earlier) suggest that two different proteins influence the expression of whiH gene in S. aureofaciens. The putative repressor-like RwhA protein protects expression of whiH in substrate mycelium either in liquid medium or during differentiation. The other detected protein, RwhB, which binds to the whiH promoter region during differentiation, may represent two forms of WhiH, one with a repressor role at the beginning of differentiation and second with the role of activator at the time of sporulation.
See more in PubMed
J Gen Microbiol. 1972 Aug;72(1):9-28 PubMed
Mol Microbiol. 1998 Jul;29(1):343-57 PubMed
Cell. 1989 Oct 6;59(1):133-43 PubMed
Microbiology (Reading). 1999 Sep;145 ( Pt 9):2229-2243 PubMed
Mol Microbiol. 1996 Aug;21(3):593-603 PubMed
Microbiology (Reading). 1998 Jun;144(6):1465-1478 PubMed
Microbiology (Reading). 2000 Nov;146 ( Pt 11):2919-2928 PubMed
J Bacteriol. 1983 Sep;155(3):1238-48 PubMed
Folia Microbiol (Praha). 1998;43(6):605-12 PubMed
J Gen Microbiol. 1976 Oct;96(2):299-315 PubMed
Can J Microbiol. 1985 May;31(5):446-50 PubMed
Gene. 1996 Nov 28;181(1-2):19-27 PubMed
Biochim Biophys Acta. 1999 Jan 18;1444(1):80-4 PubMed
Anal Biochem. 1976 May 7;72:248-54 PubMed
J Bacteriol. 2000 Aug;182(16):4606-16 PubMed
FEMS Microbiol Lett. 1997 Aug 15;153(2):371-7 PubMed
J Bacteriol. 1998 May;180(9):2515-21 PubMed
Methods Enzymol. 1980;65(1):499-560 PubMed
Mol Microbiol. 1995 Jul;17(1):37-48 PubMed
J Gen Microbiol. 1974 Apr;81(2):291-302 PubMed
Gene. 1994 May 27;143(1):101-3 PubMed
Anal Biochem. 2001 Jun 1;293(1):138-9 PubMed
