The effect of mutations in dnaK and dnaJ genes on the expression of two operons that are part of cysteine regulon was determined using Escherichia coli strains harboring cysPTWA::lacZ and cysJIH::lacZ fusions. Null dnaJ and dnaKdnaJ mutants were impaired in beta-galactosidase expression from both fusions. Efficient complementation of this defect by wild-type alleles present on a low-copy number plasmid was achieved. The presence of the pMH224 plasmid coding for CysB* protein defective in DNA binding lowered beta-galactosidase expression from cysPTWA::lacZ fusion strain harboring wild-type dnaKdnaJ alleles but did not diminish enzyme expression in delta dnaJ and delta dnaKdnaJ strains.

Department of Bacterial Genetics Institute of Microbiology Warsaw University 02 096 Warsaw Poland

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