Synthesis of chitooligomer-based glycoconjugates and their binding to the rat natural killer cell activation receptor NKR-P1
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12441671
DOI
10.1023/a:1021111703443
PII: 5108621
Knihovny.cz E-zdroje
- MeSH
- aminy chemie MeSH
- antigeny povrchové metabolismus MeSH
- buňky NK metabolismus MeSH
- chitin chemie MeSH
- chromatografie metody MeSH
- glykoproteiny chemická syntéza metabolismus MeSH
- imunoblotting MeSH
- inhibiční koncentrace 50 MeSH
- isothiokyanatany chemie MeSH
- krysa rodu Rattus MeSH
- kyselina N-acetylneuraminová metabolismus MeSH
- lektinové receptory NK-buněk - podrodina B MeSH
- lektiny typu C metabolismus MeSH
- ligandy MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- sérový albumin hovězí chemie MeSH
- skot MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- thioglykosidy chemická syntéza MeSH
- vazba proteinů MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aminy MeSH
- antigeny povrchové MeSH
- chitin MeSH
- glykoproteiny MeSH
- isothiokyanatany MeSH
- kyselina N-acetylneuraminová MeSH
- lektinové receptory NK-buněk - podrodina B MeSH
- lektiny typu C MeSH
- ligandy MeSH
- sérový albumin hovězí MeSH
- thioglykosidy MeSH
NKR-P1 protein is an important activating receptor at the surface of the rat natural killer cells. GlcNAc and chitooligomers were identified as strong activation ligands in vitro and in vivo. Their clustering brings about increase of their affinity to the NKR-P1 by 3-6 orders. Here we describe novel methodology for preparation of neoglycoproteins based on BSA carrying the chitooligomers (n = 2-5). Further on we developed novel methodology of the coupling of glycosylamines via aromatic-SCN activated linker both to protein or synthetic cores. Inhibition studies of chitooligomer glycoconjugates with the NKR-P1 receptor show that our neoglycoproteins are very strong ligands with high binding affinity (-log IC(50) = 13-15). In analogy with our previous observations with GlcNAc clustered on protein or PAMAM backbones the synthetic chitooligomer clusters should provide considerably better ligands in the in vivo antitumor treatment.
Zobrazit více v PubMed
J Biol Chem. 1994 Jun 17;269(24):16945-52 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Nature. 1994 Nov 10;372(6502):150-7 PubMed
Adv Exp Med Biol. 2001;495:343-7 PubMed
Biochem Biophys Res Commun. 1997 Sep 8;238(1):149-53 PubMed
FEBS Lett. 1998 Apr 17;426(2):243-7 PubMed
Science. 1990 Sep 14;249(4974):1298-300 PubMed
Biochem Biophys Res Commun. 2001 Sep 14;287(1):11-20 PubMed
Biochim Biophys Acta. 1964 Nov 1;83:245-55 PubMed
Curr Opin Immunol. 1995 Feb;7(1):110-20 PubMed
Nkrp1 family, from lectins to protein interacting molecules
